Refine your search
Collections
Co-Authors
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Chidrawar, Vijay R.
- Hepatoprotective Effect of Captopril on Liver Toxicity Induced by High and Low Dose of Paracetamol in Rats:Histological Study
Abstract Views :217 |
PDF Views:128
Authors
Turki M. Al-Shaikh
1,
Mahmoud M. E. Mudawi
2,
Abdelhadi Y. A. Yassin
2,
Rami S. Habeballa
2,
Vijay R. Chidrawar
2
Affiliations
1 Department of Biological Sciences, Northern Border University, Arar, SA
2 Department of Pharmacology and Toxicology, Northern Border University, SA
1 Department of Biological Sciences, Northern Border University, Arar, SA
2 Department of Pharmacology and Toxicology, Northern Border University, SA
Source
Asian Journal of Pharmaceutical Research and Health Care, Vol 8, No 3 (2016), Pagination: 92-99Abstract
Many patients may administered medications like captopril (ACE inhibitor) for treatment of chronic diseases and may also take Paracetamol as an Over The Counter (OTC) drug which may interact with captopril. Therefore, the aim of this study is to evaluate of the hepatoprotective effect of captopril on liver toxicity induced by low and high dose of paracetamol in rats. This study was conducted in two phases: first study for low dose of paracetamol (300 mg/kg); animals were divided into 4 groups of 6 rats each (n = 6); all groups were treated orally either 0.9 % Normal Saline (NS), captopril 20 mg/kg, paracetamol 300 mg/kg or captopril 20 mg/kg plus paracetamol 300 mg/kg for 10 consecutive days. Second study for single high dose of paracetamol (3000 mg/kg); animals were divided into 4 groups of 6 rats each (n = 6); all groups were pretreated orally either 0.9 % Normal Saline (NS) or captopril 20 mg/kg for 7 consecutive days followed by single oral administration of Paracetamol 3000 mg/kg or normal saline. The administration of Paracetamol or normal saline was performed 24 hours after the last administration of captopril. After 48 hours of hepatic injury induction, the animals were then sacrificed and the liver was removed for histopathological studies. Low dose (300 mg/kg) for 10 days and high single dose (3000 mg/kg) of paracetamol produced hepatotoxic effects. While captopril 20 mg/kg showed marked protection against changes induced by low and high dose of paracetamol on the liver.Keywords
Acetaminophen, Captopril, Hepatoprotective, Hepatotoxicity, Paracetamol.- Impact of Breastfeeding on Lactating Women Bone Health: A Survey Based Study in Northern Region of Saudi Arabia
Abstract Views :275 |
PDF Views:147
Authors
Affiliations
1 Northern Border University, Department of Clinical Pharmacy, Rafha, SA
2 Department of Pharmacology and Toxicology, Northern Border University, Rafha, SA
3 Northern Border University, Department of Pharmacology and Toxicology, Rafha, SA
1 Northern Border University, Department of Clinical Pharmacy, Rafha, SA
2 Department of Pharmacology and Toxicology, Northern Border University, Rafha, SA
3 Northern Border University, Department of Pharmacology and Toxicology, Rafha, SA
Source
Asian Journal of Pharmaceutical Research and Health Care, Vol 9, No 4 (2017), Pagination: 158-166Abstract
Bone fitness is considered as an important public health problem especially in post-menopausal women. Numerous studies have investigated to understand beneficiary effects of mother milk on the baby but very few studies have been conducted to understand the effect of breastfeeding on the bone health of lactating women. We hypothesized that high calcium demand during pregnancy and lactation and low estrogenic state support may affect on the bone health immediately after child birth and later in the life. Based upon this theory we have conducted a survey based study in the Northern region of Saudi Arabia to assess the health status of the lactating mothers. Total 376 lactating mothers were interviewed during the period of 16 Nov 2016 to 23 March 2017. A pre-tested and structured questionnaire was provided at their homes to collect information. The result revealed that among 376 participants, 7% complaining about generalized pain, 41% suffering from joint pain, 24%, suffering from lower back pain and 1% of mothers suffered from fracture. This survey highlights important but ignored aspect of the bone health of lactating women in the Northern region of Saudi Arabia. During pregnancy, women consume calcium supplements to satisfy their requirement of developing baby but after child birth, they stop calcium supplement though it's needed. Based upon this study we state that there is a need to prepare and follow guidelines for the use of calcium supplement for the lactating women to maintain bone health.Keywords
Bone Health, Estrogen and Calcium, Lactation, Pregnancy.References
- Ramesh K, Kumar KP. Knowledge and attitude of post natal mothers towards breast feeding in a tertiary care hospital, Bellary, Karnataka. International J Sci Res. 2014; 3(4):368– 70.
- WHO, Infant and young child feeding Model Chapter for textbooks for medical students and allied health professionals. Available from: Crossref
- Kovacs CS. Calcium and bone metabolism in pregnancy and lactation. Endocrinol Metab Clin North Am. 2011 Dec; 40(4):795–826. Crossref PMid:22108281
- Oliveri B, Parisi MS, Zeni S, Mautalen C. Mineral and bonemass changes during pregnancy and lactation. Nutrition. 2004 Feb; 20(2):235–40. Crossref PMid:14962693
- Prentice A. Calcium in pregnancy and lactation. Annu Rev Nutr. 2000; 20:249–72. Crossref PMid:10940334
- El-Zorkany. The Middle East and Africa regional audit. International Osteoporosis Foundation; 2013.
- Hreshchyshyn MM, Hopkins A, Zylstra S, Anbar M. Associations of parity, breastfeeding, and birth control pills with lumbar spine and femoral neck bone densities. Am J Obstet Gynecol. 1988 Aug; 159(2):318–22. Crossref
- Lopez JM, Gonzalez G, Reyes V, Campino C, Díaz S. Bone turnover and density in healthy women during breastfeeding and after weaning. Osteoporos Int. 1996; 6(2):153–9. Crossref PMid:8704355
- Kovacs CS, Kronenberg HM. Maternal-fetal calcium and bone metabolism during pregnancy, puerperium and lactation. Endocr Rev. 1997 Dec; 18(6):832–72. Crossref PMid:9408745
- Kirby BJ, Ardeshirpour L, Woodrow JP, Wysolmerski JJ, Sims NA, Karaplis AC, Kovacs CS. Skeletal recovery after weaning does not require PTHrP. J Bone Miner Res. 2011 Jun; 26(6):1242–51. Crossref PMid:21308774 PMCid:PMC3179289
- Wysolmerski JJ. Conversations between breast and bone: physiological bone loss during lactation as evolutionary template for osteolysis in breast cancer and pathological bone loss after menopause. BoneKEy. 2007 Aug; 4(8):209– 25. Crossref
- Kovacs CS. The role of vitamin D in pregnancy and lactation: Insights from animal models and clinical studies. Annu Rev Nutr. 2012 Aug 21; 32:97–123. Crossref PMid:22483092
- Holmberg-Marttila D, Sievanen H, Tuimala R. Changes in bone mineral density during pregnancy and postpartum: prospective data on five women. Osteoporos Int. 1999; 10(1):41–6. Crossref PMid:10501778
- Naylor KE1, Iqbal P, Fledelius C, Fraser RB, Eastell R. The effect of pregnancy on bone density and bone turnover. J Bone Miner Res. 2000 Jan; 15(1):129–37. Crossref PMid:10646122
- Pearson D, Kaur M, San P, Lawson N, Baker P, Hosking D. Recovery of pregnancy mediated bone loss during lactation. Bone. 2004 Mar; 34(3):570–8. Crossref PMid:15003805
- Saleh M, Kerr M. Understanding the Muslim patient. Journal of the Society of Obstetricians and Gynecologists of Canada. 1996; 18:55–64. Crossref
- Lippuner K, Zehnder HJ, Casez JP, Takkinen R, Jaeger P. Effects of PTH-related protein (PTH-rP) on calcium-phosphate metabolism in nursing mothers. Bone. 1995; 16(Suppl 1):209.
- Qur’an, 2: 233.
- Kalkwarf HJ, Specker BL, Bianchi DC, Ranz J, Ho M. The effect of calcium supplementation on bone density during lactation and after weaning. N Engl J Med. 1997 Aug 21; 337(8):523–8. Crossref PMid:9262495
- Kalkwarf HJ, Specker BL Bone mineral changes during pregnancy and lactation. Endocrine. 2002 Feb; 17(1):49– 53. Crossref
- Ulrich U1, Miller PB, Eyre DR, Chesnut CH 3rd, Schlebusch H, Soules MR. Bone remodelling and bone mineral density during pregnancy. Arch Gynecol Obstet. 2003 Oct; 268(4):309–16. Crossref PMid:14504876
- Kaur M1, Pearson D, Godber I, Lawson N, Baker P, Hosking D. Longitudinal changes in bone mineral density during normal pregnancy. Bone. 2003 Apr; 32(4):449–54. Crossref
- Kent GN, Price RI, Gutteridge DH, Allen JR, Rosman KJ, Smith M, Bhagat CI, Wilson SG, Retallack RW. Effect of pregnancy and lactation on maternal bone mass and calcium metabolism. Osteoporos Int. 1993; 3(Suppl 1):44–7. Crossref PMid:8461575
- Kohlmeier L, Marcus R. Calcium disorders of pregnancy. Endocrinol Metab Clin North Am. 1995 Mar; 24(1):15–39. PMid:7781623
- Karlsson MK1, Ahlborg HG, Karlsson C. Female reproductive history and the skeleton- A review. BJOG. 2005 Jul; 112(7):851–6. Crossref PMid:15957983.
- Comparative Experimental Studies of Few L-Type and T-Type Ca2+ Channel Blockers against In-Ovo and In-Vitro Models of Angiogenesis
Abstract Views :266 |
PDF Views:121
Authors
Affiliations
1 Department of Pharmacology and Toxicology, Northern Border University, Rafha, SA
2 Department of Pharmaceutical Chemistry, Northern Border University, Rafha, SA
1 Department of Pharmacology and Toxicology, Northern Border University, Rafha, SA
2 Department of Pharmaceutical Chemistry, Northern Border University, Rafha, SA
Source
Asian Journal of Pharmaceutical Research and Health Care, Vol 10, No 1 (2018), Pagination: 1-9Abstract
Angiogenesis is the development of new blood vessels from pre-existing one. The ion-channels on endothelium plays vital role in cell proliferation and related angiogenesis. We aimed to investigate the effects of L-type (Verapamil and Diltiazem) and T-type (Ethosuximide) Calcium Channel Blockers (CCBs) on neovascularization. The effects on neovascularization were investigated by in-ovo (CAM, Chick Chorioallantoic membrane) and in-vitro (aortic ring assay) methods. Each test drug was tested for at least 3 doses and the anti-angiogenic effect was compared with Suramin as standard and normal control groups. Various vital parameters were recorded during the experiment like the number of blood capillaries, sprouts formation, angiogenic score etc. The L-type Ca2+ channel blockers Verapamil at the dose of 50μM, 110 and 220 μM/disk has shown significant (p < 0.001) reduction in the number of branching points in CAM assay. For the further confirmation, angiogenic activity was evaluated in vitro by rat aortic ring assay method; the area of sprouts was reduced by the medium and high dose of verapamil. Diltiazem has demonstrated modest anti-angiogenic activity by both the models, whereas T-type calcium channel blocker ethosuximide has not shown any effect on the neovascularization. Among all the tested drugs verapamil has shown the promising anti-angiogenic property. Thus verapamil and diltiazem may have antiangiogenic activity defines novel beneficial effects in angiogenic mediated pathological conditions in addition to their main indications in cardiovascular complications.Keywords
Angiogenesis, CAM Assay, L-Type Ca2+ Channel Blockers, Rat Aortic Ring Assay, T-Type Calcium Channel Blocker.References
- Folkman J, Klagsbrun M. Angiogenic factors. Science. 1987 Jan 23; 235(4787):442–7. crossref PMid:2432664
- Folkman J. Seminars in medicine of the beth israel hospital, Boston. Clinical applications of research on angiogenesis. N Engl J Med. 1995 Dec 28; 333(26):1757–63. crossref PMid:7491141
- Otrock ZK, Mahfouz RA, Makarem JA, Shamseddine AI. Understanding the biology of angiogenesis: Review of the most important molecular mechanisms. Blood Cells Mol Dis. 2007 Sep-Oct; 39(2):212–20. crossref PMid:17553709
- Tahergorabi Z, Khazaei M. A review on angiogenesis and its assays. Iran J Basic Med Sci. 2012 Nov; 15(6):1110–26. PMid:23653839 PMCid:PMC3646220
- Deryugina EI, Quigley JP. Tumor angiogenesis: MMP-mediated induction of intravasation- and metastasis-sustaining neovasculature. Matrix Biol. 2015 May-Jul; 44-46:94–112. crossref PMid:25912949 PMCid:PMC5079283
- D’Amore PA, Thompson RW. Mechanisms of angiogenesis. Annu Rev Physiol. 1987; 49:453–64. crossref PMid:2436570
- Augustin HG. Antiangiogenic tumour therapy: will it work? Trends Pharmacol Sci. 1998 Jun; 19(6):216–22. crossref
- Balk SD. Calcium as a regulator of the proliferation of normal, but not of transformed, chicken fibroblasts in a plasma-containing medium. Proc Natl Acad Sci U S A. 1971 Feb; 68(2):271–5. crossref PMid:5277067 PMCid:PMC388915
- Boynton AL, Whitfield JF, Isaacs RJ, Morton HJ. Control of 3T3 cell proliferation by calcium. In Vitro. 1974 Jul-Aug; 10:12–7. crossref PMid:4471173
- Durham AC, Walton JM. Calcium ions and the control of proliferation in normal and cancer cells. Biosci Rep. 1982 Jan; 2(1):15–30. crossref PMid:7037065
- Boynton AL, Whitfield JF. Different calcium requirements for proliferation of conditionally and unconditionally tumorigenic mouse cells. Proc Natl Acad Sci U S A. 1976 May; 73(5):1651–4. crossref PMid:1064038 PMCid:PMC430357
- Boynton AL, Whitfield JF, Isaacs RJ, Tremblay RG. Different extracellular calcium requirements for proliferation of nonneoplastic, preneoplastic, and neoplastic mouse cells. Cancer Res. 1977 Aug; 37(8 Pt 1):2657–61. PMid:872093
- Parsons PG. Selective proliferation of human tumour cells in calcium-depleted medium. Aust J ExpBiol Med Sci. 1978 Jun; 56(3):297–300. crossref PMid:101190
- Paul D, Ristow HJ. Cell cycle control by Ca++-ions in mouse 3T3 cells and in transformed 3T3 cells. J Cell Physiol. 1979 Jan; 98(1):31-9. crossref PMid:762200
- Lokman NA, Elder AS, Ricciardelli C, Oehler MK. Chick Chorioallantoic Membrane (CAM) assay as an in vivo model to study the effect of newly identified molecules on ovarian cancer invasion and metastasis. Int J Mol Sci. 2012; 13(8):9959–70. crossref PMid:22949841 PMCid:PMC3431839
- Burgermeister J1, Paper DH, Vogl H, Linhardt RJ, Franz G. LaPSvS1, a (1-->3)-beta-galactan sulfate and its effect on angiogenesis in vivo and in vitro. Carbohydr Res. 2002 Sep 9; 337(16):1459–66. crossref
- Krenn L, Paper DH. Inhibition of angiogenesis and inflammation by an extract of red clover (Trifoliumpratense L.). Phytomedicine. 2009 Dec; 16(12):1083–8. crossref PMid:19665361
- AlMalki WH, Shahid I, Mehdi AY, Hafeez MH. Assessment methods for angiogenesis and current approaches for its quantification. Indian J Pharmacol. 2014 May-Jun; 46(3):251–6. crossref PMid:24987169 PMCid:PMC4071699
- Fernandes G, Barone A, Dziak R. Effects of verapamil on bone cancer cells In Vitro. J Cell Biol Cell Metab. 2016; 3:013.
- Caglar Y, Ali Cetin, Demirci F, Zubeyde AP, Tuba K, Ahmet A, Meral C, Ozlem K Y, Ismihan G. Anti-angiogenic effects of diltiazem, imatinib, and bevacizumab in the CAM assay. International Journal of Scientific and Research Publications. 2013; 3(8).
- Gomora JC1, Daud AN, Weiergräber M, Perez-Reyes E. Block of cloned human T-type calcium channels by succinimide antiepileptic drugs. Mol Pharmacol. 2001 Nov; 60(5):1121–32. crossref PMid:11641441
- Kerbel R, Folkman J. Clinical translation of angiogenesis inhibitors. Nat Rev Cancer. 2002 Oct; 2(10):727–39. crossref PMid:12360276
- Fidler IJ. The pathogenesis of cancer metastasis: the ‘seed and soil’ hypothesis revisited. Nat Rev Cancer. 2003 Jun; 3(6):453–8. crossref PMid:12778135
- Bergers G, Benjamin LE. Tumorigenesis and the angiogenic switch. Nat Rev Cancer. 2003 Jun; 3(6):401–10. crossref PMid:12778130
- Capiod T. Cell proliferation, calcium influx and calcium channels. Biochimie. 2011 Dec; 93(12):2075–9. crossref PMid:21802482
- Harris-Hooker SA, Gajdusek CM, Wight TN, Schwartz SM. Neovascular responses induced by cultured aortic endothelial cells. J Cell Physiol. 1983 Mar; 114(3):302–10. crossref PMid:6187756
- Nguyen M, Shing Y, Folkman J. Quantitation of angiogenesis and antiangiogenesis in the chick embryo chorioallantoic membrane. Microvasc Res. 1994 Jan; 47(1):31–40. crossref PMid:7517489
- Lam CF, Liu YC, Tseng FL, Sung YH, Huang CC, Jiang MJ, Tsai YC. High-dose morphine impairs vascular endothelial function by increased production of superoxide anions. Anesthesiology. 2007 Mar; 106(3):532–7. crossref PMid:17325512
- Mason RP1, Mak IT, MW Trumbore, Mason PE. Antioxidant properties of calcium antagonists related to membrane biophysical interactions. Am J Cardiol. 1999 Aug 19; 84(4A):16L–22L. PMid:10480441
- Hayman SR, Leung N, Grande JP, Garovic VD. VEGF inhibition, hypertension, and renal toxicity. Curr Oncol Rep. 2012 Aug; 14(4):285–94. crossref PMid:22544560 PMCid:PMC3746763
- Nicosia RF, Ottinetti A. Growth of microvessels in serumfree matrix culture of rat aorta. A quantitative assay of angiogenesis in vitro. Lab Invest. 1990 Jul; 63(1):115–22. PMid:1695694
- Nicosia RF, Villaschi S. Autoregulation of angiogenesis by cells of the vessel wall. Int Rev Cytol. 1999; 185:1–43. crossref
- Mariot P, Vanoverberghe K, Lalevee N, Rossier MF, Prevarskaya N. Overexpression of an alpha 1H (Cav3.2) T-type calcium channel during neuroendocrine differentiation of human prostate cancer cells. J Biol Chem. 2002 Mar 29; 277(13):10824-33. Epub 2002 Jan 17. crossref PMid:11799114
- Ciapa B, Pesando D, Wilding M, Whitaker M. Cell-cycle calcium transients driven by cyclic changes in inositol trisphosphate levels. Nature. 1994 Apr 28; 368(6474):875–8. crossref PMid:8159248
- Choi DW. Ionic dependence of glutamate neurotoxicity. J Neurosci. 1987 Feb; 7(2):369–79. PMid:2880938